Recombinant I-309/CCL1, Human

• Catalog Number: orb1494846
• Category: Proteins
• Description: Chemokine (C-C motif) ligand 1 (CCL1), also known as I-309, is a small glycoprotein secreted by activated T cells that belongs to the family ofchemokines. Human CCL1 has been assumed to be a homologue of mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. CCL1 attracts monocytes, NK cells, immature B cells and dendritic cells by interacting with the cell surface chemokine receptor CCR8. This chemokine resides in a large cluster of CC chemokines on human chromosome 17.Recombinant Human I-309/CCL1 produced in E.coli is a single non-glycosylated polypeptide chain containing 74 amino acids. A fully biologically active molecule, rhI-309/CCL1has a molecular mass of 8.5 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• MW: 8.5 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: SKS MQVPFSRCCF SFAEQEIPLR AILCYRNTSS ICSNEGLIFK LKRGKEACAL DTVGWVQRHR KMLRHCPSKR K
• Source: Escherichia coli.
• Biological Activity: The EC50 value of human I-309/CCL on Caˆ2+ mobilization assay in CHO-K1/Gα15/hCCR8 cells (human Gα15 and human CCR8 stably expressed in CHO-K1 cells) is less than 1 μg/ml.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant human I-309°CCL1 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, recombinant human I-309°CCL1 should be stable up to 1 week at 4°C or up to 2 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Alternative names: CCL1, TCA-3
• Note: For research use only
• Application notes: Reconstituted in ddH2O or PBS at 100 μg/ml.
• Expiration Date: 6 months from date of receipt.