Recombinant human/Cyn. monkey/rhesus macaque CD28 protein, N-His-Avi (HEK293)

• Catalog Number: orb1516480
• Category: Proteins
• Description: Recombinant human/Cyn. monkey/rhesus macaque CD28 protein, N-His-Avi (HEK293)
• Tag: N-His-Avi
• Concentration: > 0.5 mg/ml
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
• MW: Due to glycosylation, the protein migrates to 36-52 kDa based on Tris-Bis PAGE result. kDa
• Target: T-cell-specific surface glycoprotein CD28
• Protein Sequence: 17-152/220
• Expression System: HEK293 cell
• Endotoxins: < 1.0 EU/μg as determined by LAL
• Storage: Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles.
• Alternative names: CD 28; CD28 antigen; CD28 molecule; MGC138290; T c
• Note: For research use only
• Application notes: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Expiration Date: 6 months from date of receipt.

Human /Cynomolgus / Rhesus macaque CD28 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Immobilized Human /Cynomolgus / Rhesus macaque CD28, His Tag at 0.5 µg/ml (100 µl/Well) on the plate. Dose response curve for Anti-CD28 Ab, hFc Tag with the EC50 of 18.8 ng/ml determined by ELISA.

Immobilized Human /Cynomolgus / Rhesus macaque CD28, His Tag at 0.5 µg/ml (100 µl/Well) on the plate. Dose response curve for Anti-CD28 Ab, hFc Tag with the EC50 of 18.8/16.1/26.2 ng/ml determined by ELISA.

The purity of Human / Cynomolgus / Rhesus macaque CD28 was greater than 95% as determined by SEC-HPLC.