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Catalog Number | orb1088542 |
---|---|
Category | Proteins |
Description | Recombinant Human Vascular Endothelial Growth Factor (VEGF165) protein is produced by Mammalian expression system and the target gene encoding Ala27-Arg191 is expressed with a his tag at the C-terminus. The VEGF165 protein has a calculated molecular mass of 19.8 kDa and migrates as an 24-27 kDa band in SDS-PAGE under reducing conditions due to glycosylation. Accession Number: P15692-4 Also known as: VEGFA、VPF |
Tested applications | CLIA, EIA, LF |
Tag | His-tag |
Concentration | Lot specific(±20%) |
Form/Appearance | Supplied as a 0.2 μm filtered solution of PBS, pH 7.4 |
Purity | > 90% as determined by SDS-PAGE |
MW | 20.8 kDa |
Target | VEGFA |
UniProt ID | P15692 |
Source | HEK293 |
Expression System | HEK293 Cells |
Storage | Short term (up to 7 days) store at 2–8°C. Long term, store at -20°C. Avoid multiple freeze/thaw cycles. |
Buffer/Preservatives | PBS,20%Glycerol,pH 7.4 |
Note | For research use only |
Application notes | Use: Calibration/quality control. Quality control: Mol Mass: 19.8 kDa AP Mol Mass: 24-27 kDa Purity: ≧90% as determined by reducing SDS-PAGE |
Expiration Date | 6 months from date of receipt. |
> 95% as determined by SDS-PAGE | |
20 kDa |
> 90% as determined by SDS-PAGE | |
25 kDa |
98.00% | |
86.2 kDa (predicted). Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result. |