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Catalog Number | orb1516905 |
---|---|
Category | Proteins |
Description | Recombinant human VEGF165 protein, C-His |
Tag | C-His |
Concentration | > 0.5 mg/ml |
Purity | > 95% as determined by SDS-PAGE |
MW | 20 kDa |
Target | Vascular endothelial growth factor A |
Protein Sequence | 27-191/191 |
Expression System | E.coli |
Endotoxins | Not analyzed |
Storage | Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles. |
Alternative names | VEGF 165; Recombinant VEGF 165A; Vascuoar endothel Read more... |
Note | For research use only |
Application notes | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
CLIA, EIA, LF | |
> 90% as determined by SDS-PAGE | |
20.8 kDa | |
HEK293 |
98.00% | |
17 kDa (predicted). Due to glycosylation, the protein migrates to 18 kDa and 22-25 kDa under reduced (R) condition, 30 kDa and 32-40 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
98.00% | |
17 kDa (predicted). Due to glycosylation, the protein migrates to 18 kDa and 22-25 kDa under reduced (R) condition, 30 kDa and 32-40 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
> 95% | |
22.2 kDa (predicted). Due to glycosylation, the protein migrates to 28-35 kDa under reduced (R) condition, 45-60 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
> 95% | |
22.2 kDa (predicted). Due to glycosylation, the protein migrates to 28-33 kDa under reduced (R) condition, 45-55 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |