Recombinant human TNFR2 protein, C-mFc (HEK293)

• Catalog Number: orb1516309
• Category: Proteins
• Description: Recombinant human TNFR2 protein, C-mFc (HEK293)
• Tag: C-mFc
• Concentration: > 0.5 mg/ml
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
• MW: Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result. kDa
• Target: Tumor necrosis factor receptor superfamily member 1B
• Protein Sequence: 23-257/461
• Expression System: HEK293 cell
• Endotoxins: < 1.0 EU/μg as determined by LAL
• Storage: Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles.
• Alternative names: TNF receptor-associated factor 2; E3 ubiquitin-pro
• Note: For research use only
• Application notes: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Expiration Date: 6 months from date of receipt.

Human TNFR2 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

Immobilized Human TNFR2, mFc Tag at 0.5 µg/ml (100 µl/Well). Dose response curve for Anti-TNFR2 Ab. with the EC50 of 12.0 ng/ml determined by ELISA.

Immobilized Human TNFR2, mFc Tag at 5 µg/ml (100 µl/Well). Dose response curve for Human TNF alpha, His Tag with the EC50 of 0.2 µg/ml determined by ELISA.

The purity of Human TNFR2 is greater than 95% as determined by SEC-HPLC.