Recombinant human TIGIT protein, C-hFc (HEK293)

• Catalog Number: orb1516315
• Category: Proteins
• Description: Recombinant human TIGIT protein, C-hFc (HEK293)
• Tag: C-hFc
• Concentration: > 0.5 mg/ml
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
• MW: Due to glycosylation, the protein migrates to 48-50 kDa based on Tris-Bis PAGE result. kDa
• Target: T-cell immunoreceptor with Ig and ITIM domains
• Protein Sequence: 22-141/244
• Expression System: HEK293 cell
• Endotoxins: < 1.0 EU/μg as determined by LAL
• Storage: Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles.
• Alternative names: TIGIT; VSIG9; VSTM3; WUCAM; T cell immunoreceptor
• Note: For research use only
• Application notes: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Expiration Date: 6 months from date of receipt.

Immobilized Biotinylated Human CD155, His Tag at 1 µg/ml (100 µl/Well) on Streptavidin precoated (5 µg/well) plate. Dose response curve for Human TIGIT, hFc Tag with the EC50 of 5.3 ng/ml determined by ELISA.

Immobilized Biotinylated Human Nectin-2, His Tag at 5 µg/ml (100 µl/Well) on Streptavidin precoated (5 µg/ml) plate. Dose response curve for Human TIGIT, hFc Tag with the EC50 of 24.48 µg/ml determined by ELISA.

Recombinant Human TIGIT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Human TIGIT is greater than 95% as determined by SEC-HPLC.