Recombinant human SIGLEC3 protein, C-hFc (HEK293)

• Catalog Number: orb1516335
• Category: Proteins
• Description: Recombinant human SIGLEC3 protein, C-hFc (HEK293)
• Tag: C-hFc
• Concentration: ＞0.5 mg/ml
• Form/Appearance: Lyophilized
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
• MW: Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result.
• Target: Myeloid cell surface antigen CD33
• Protein Sequence: 18-259/364
• Expression System: HEK293 cell
• Biological Activity: Not tested
• Endotoxins: < 1.0 EU/μg as determined by LAL
• Storage: Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.
• Alternative names: CD33 antigen (gp67); CD33 antigen; CD33 molecule;
• Note: For research use only
• Application notes: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Expiration Date: 6 months from date of receipt.

Immobilized Human CD33, Fc at 0.5 µg/ml (100 µl/Well) on plate. Dose response curve for biotinylated Anti-CD33 Ab. with the EC50 of 35 ng/ml determined by ELISA.

Recombinant siglec 3 Protein on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

The purity of Siglec 3 Protein is greater than 95% as determined by SEC-HPLC.