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Catalog Number | orb1516359 |
---|---|
Category | Proteins |
Description | Recombinant human PD-1 protein, C-His (HEK293) |
Tag | C-His |
Concentration | > 0.5 mg/ml |
Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC |
MW | Due to glycosylation, the protein migrates to 35-55 kDa based on Tris-Bis PAGE result. kDa |
Target | Programmed cell death protein 1 |
Protein Sequence | 25-167/288 |
Expression System | HEK293 cell |
Endotoxins | < 1.0 EU/μg as determined by LAL |
Storage | Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles. |
Alternative names | Programmed cell death protein 1; CD279; CD279 anti Read more... |
Note | For research use only |
Application notes | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Expiration Date | 6 months from date of receipt. |
Immobilized Human PD-1, His Tag at 0.5 µg/ml (100 ul/Well). Dose response curve for Anti-PD-1 Ab. with the EC50 of 45.0 ng/ml determined by ELISA.
Immobilized Human PD-1, His Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Anti-PD-1 Ab. with the EC50 of 45.0/41.6/45.7 ng/ml determined by ELISA.
Recombinant PD-1 on Tris-Bis PAGE under reduced. The purity is greater than 95%.
The purity of PD-1 is greater than 95% as determined by SEC-HPLC.
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 40-52 kDa based on Tris-Bis PAGE result. kDa |
98.00% | |
14.8 kDa (predicted). Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result. |
98.00% | |
14.6 kDa (predicted). Due to glycosylation, the protein migrates to 25-35 kDa based on Tris-Bis PAGE result. |
98.00% | |
25.3 kDa (predicted). Due to glycosylation, the protein migrates to 40-52 kDa based on Tris-Bis PAGE result. |