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Catalog Number | orb1905934 |
---|---|
Category | Proteins |
Description | Recombinant Human Osteopontin/OPN |
Form/Appearance | Lyophilized from 0.2 μm filtered concentrated solution in PBS, pH 7.2. |
Purity | > 95 % by SDS-PAGE analyses. |
MW | Apparent molecular mass of 60-65 kDa in SDS-PAGE under reducing conditions, a single glycosylated polypeptide protein consisting of 290 amino acids including a C-terminal 6x polyhistidine tag. |
Protein Sequence | Ile17-Asn300, with a C-terminal 6-His tag; Accession # NP_000573.1 |
Source | Mouse myeloma cell line, NS0 |
Expression System | Mouse myeloma cell line, NS0 |
Biological Activity | Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x105 cells/well are added to a rHuOsteopontin/OPN coated plate, cell adhesion is enhanced in a dosedependent manner after 1 hour incubation at 37˚C. The ED50 for this effect is typically 0.1-0.6 μg/mL. rHuThrombin proteolytic treatment of this rHuOsteopontin can increase HEK293 cell adhesion by about 5-fold. |
Endotoxins | Less than 0.1 EU/ug of rHuOsteopontin/OPN as determined by LAL method. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
80% | |
33.0 kDa | |
Human Osteopontin, His Tag (orb257724) is expressed from human 293 cells (HEK293). It contains AA Ile 17 - Asn 300 (Accession # AAH07016). |
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