Recombinant Human Noggin

Catalog Number: orb1906230

Catalog Number	orb1906230
Category	Proteins
Description	Recombinant Human Noggin
Form/Appearance	Lyophilized from a 0.2 um filtered concentrated solution in PBS.
Purity	> 95 % by SDS-PAGE and HPLC analyses.
MW	Approximately 46.3 kDa, non-disulfide-linked homodimer consisting of two 206 amino acid polypeptide chains.
UniProt ID	Q13253
Protein Sequence	MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGAAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC
Source	Escherichia coli
Expression System	Escherichia coli
Biological Activity	Fully biologically active when compared to standard. The ED50 as determined by inhibiting BMP-4-induced alkaline phosphatase production of murine ATDC5 cells is less than 3.0 ng/ml, corresponding to a specific activity of > 3.3 �� 105 IU/mg in the presence of 5 ng/ml rHuBMP-4.
Endotoxins	Less than 1 EU/ug of rHuNoggin as determined by LAL method.
Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Note	For research use only
Expiration Date	6 months from date of receipt.

Purity:	> 95 % by SDS-PAGE and HPLC analyses.
MW:	Approximately 23.0 kDa on SDS-PAGE under reducing conditions, containing 205 amino acids, and a molecular mass about 47.9 kDa homodimer under non-reducing conditions (Molecular size on SDS-PAGE will appear at approximately 50-80 kDa).
Source:	Insect Cell

Sizes Available: 100 μg, 5 μg, 500 μg

Purity:	> 95% by Tris-Bis PAGE
Source:	KMP2049, Recombinant Human Noggin Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Gln28-Cys232) of Human Noggin fused with no tag .

Sizes Available: 500 μg, 100 μg

Purity:	> 85% by SDS-PAGE.
Source:	KMP1354, Recombinant human Noggin Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Gln28-Cys232) of human Noggin (Accession #NP_005441.1) fused with a 6×His Tag at the C-terminus.

Sizes Available: 50 μg, 100 μg

Purity:	Greater than 85% as determined by SDS-PAGE.
MW:	58.2 kDa
Source:	E.coli

Conjugation:	Unconjugated
Purity:	Greater than 95% as determined by reducing SDS-PAGE.
MW:	50.2 KDa
Source:	Mammalian