Recombinant Human Melanoma Inhibitor Activity Protein 2 (rHuMIA-2)

• Catalog Number: orb1494951
• Category: Proteins
• Description: MIA2 is a secreted cytokine and a member of the MIA/OTOR family. Members of this family which also includes MIA, OTOR, and TANGO share a Src homology-3 (SH3)-like domain. MIA2 is predominantly expressed in hepatocytes. Elevated levels of MIA2 may represent a clinically useful marker for diagnosis of hepatic disease activity and severity.
• Form/Appearance: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl.
• Purity: > 97% by SDS-PAGE and HPLC analyses.
• MW: 11.5 kDa, a single non-glycosylated polypeptide chain containing 101 amino acids.
• Protein Sequence: MLESTKLLAD LKKCGDLECE ALINRVSAMR DYRGPDCRYL NFTKGEEISV YVKLAGERED LWAGSKGKEF GYFPRDAVQI EEVFISEEIQ MSTKESDFLC L
• Source: Escherichia coli
• Biological Activity: Data Not Available.
• Endotoxins: Less than 1EU/µg of rHuMIA-2 as determined by LAL method.
• Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl.
• Note: For research use only
• Application notes: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.
• Expiration Date: 6 months from date of receipt.