Recombinant human LIGHT (Trimer) protein, N-His-Flag (HEK293)

• Catalog Number: orb1516393
• Category: Proteins
• Description: Recombinant human LIGHT (Trimer) protein, N-His-Flag (HEK293)
• Tag: N-His-Flag
• Concentration: ＞0.5 mg/ml
• Form/Appearance: Lyophilized
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
• MW: Due to glycosylation, the protein migrates to 52-140 kDa based on Tris-Bis PAGE result.
• Target: Tumor necrosis factor ligand superfamily member 14
• Protein Sequence: 74-240/240
• Expression System: HEK293 cell
• Biological Activity: Not tested
• Endotoxins: < 1.0 EU/μg as determined by LAL
• Storage: Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.
• Alternative names: Tumor necrosis factor ligand superfamily member 14
• Note: For research use only
• Application notes: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Expiration Date: 6 months from date of receipt.

Immobilized Human HVEM at 0.5 ug/ml (100 ul/Well). Dose response curve for Human Trimer LIGHT, with the EC50 of 0.24 ug/ml determined by ELISA.

Recombinant LIGHT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of LIGHT is greater than 95% as determined by SEC-HPLC.