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Catalog Number | orb1906287 |
---|---|
Category | Proteins |
Description | Recombinant Human Keratinocyte Growth Factor-2/FGF-10 |
Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in 2 x PBS, pH 7.4. |
Purity | > 97 % by SDS-PAGE and HPLC analyses. |
MW | Approximately 19.1 kDa, a single, non-glycosylated polypeptide chain containing 169 amino acids. |
UniProt ID | O15520 |
Protein Sequence | LGQDMVSPEA TNSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS |
Source | Escherichia coli |
Expression System | Escherichia coli |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x106 IU/mg. |
Endotoxins | Less than 1 EU/ug of rHuKGF-2/FGF-10 as determined by LAL method. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
HPLC, SDS-PAGE | |
Unconjugated | |
> 95% by SDS-PAGE and HPLC analyses | |
19.5 kDa |
> 90% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
KMP1961, Recombinant Human FGF10 Protein is produced by E.coli expression system. The target protein is expressed with sequence (Gln38-Ser208) of Human FGF10 fused with no tag . |
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