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Catalog Number | orb2658095 |
---|---|
Category | Proteins |
Description | Recombinant Human Interleukin-4 (IL4) (Active) |
Tag | C-terminal 10xHis-tagged |
Form/Appearance | Lyophilized powder |
Purity | Greater than 90% as determined by SDS-PAGE. |
MW | 16.3 kDa |
UniProt ID | P05112 |
Protein Sequence | HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS |
Protein Length | Full Length of Mature Protein |
Source | Mammalian cell |
Biological Origin | Homo sapiens (Human) |
Biological Activity | The ED50 as determined by the dose-dependent stimulation of the proliferation of TF-1 cells is 1.864-4.949 ng/mL. |
Expression Region | 25-153aa |
Endotoxins | Less than 1.0 EU/ug as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
Buffer/Preservatives | Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4 |
Alternative names | Interleukin-4; IL-4; B-cell stimulatory factor 1 ( Read more... |
Note | For research use only |
Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Expiration Date | 6 months from date of receipt. |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
The ED50 as determined by the dose-dependent stimulation of the proliferation of TF-1 cells is 1.864-4.949 ng/mL.
> 98% as determined by SDS-PAGE and HPLC. | |
15.0 kDa | |
E.Coli |
Greater than 95% as determined by SDS-PAGE. | |
15.1 kDa | |
E.coli |
Greater than 95% as determined by SDS-PAGE. | |
14.97 kDa | |
Mammalian cell |
Greater than 95% as determined by SDS-PAGE. | |
16 kDa | |
Mammalian cell |
Greater than 95% as determined by SDS-PAGE. | |
24.4 kDa | |
Mammalian cell |