Recombinant Human Interleukin-10 (rHuIL-10)

• Catalog Number: orb1495047
• Category: Proteins
• Description: Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of α-helical cytokines that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 . IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts . Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells . IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer . The IL-10 dimer binds to two IL-10 Rα/IL-10R1 chains, resulting in recruitment of two IL-10 Rβ/IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 . IL-10Rβ does not bind IL-10 by itself but is required for signal transduction . IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation . It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses.
• Form/Appearance: Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
• Purity: > 95% by SDS-PAGE and HPLC analyses.
• MW: Approximately 18.6kDa, a single non-glycosylated polypeptide chain containing 161 amino acids.
• Protein Sequence: MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLKESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N
• Source: Escherichia coli.
• Biological Activity: Determined by the dose-dependent co-stimulation (with murine IL-4) of MC/9 cells. The ED50 was found to be ≤ 2 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.
• Endotoxins: Less than 1EU/mg of rHuIL-10 as determined by LAL method.
• Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
• Note: For research use only
• Application notes: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.
• Expiration Date: 6 months from date of receipt.