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Catalog Number | orb1906278 |
---|---|
Category | Proteins |
Description | Recombinant Human Insulin-like Growth Factor-Binding Protein 4, Insect Cells Derived |
Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in 20mM Tris-HCl, pH 8.0, 150mM NaCl. |
Purity | > 97 % by SDS-PAGE and HPLC analyses. |
MW | The apparent molecular weight is about 30 kDa in SDS-PAGE under reducing conditions, containing 237 amino acids. |
Protein Sequence | DEAIHCPPCS EEKLARCRPP VGCEELVREP GCGCCATCAL GLGMPCGVYT PRCGSGLRCY PPRGVEKPLH TLMHGQGVCM ELAEIEAIQE SLQPSDKDEG DHPNNSFSPC SAHDRRCLQK HFAKIRDRST SGGKMKVNGA PREDARPVPQ GSCQSELHRA LERLAASQSR THEDLYIIPI PNCDRNGNFH PKQCHPALDG QRGKCWCVDR KTGVKLPGGL EPKGELDCHQ LADSFRE |
Source | Insect Cell |
Expression System | Insect Cell |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 cells is less than 0.1 μg/ml, corresponding to a specific activity of > 1.0 x104 IU/mg in the presence of 14 ng/ml of rHuIGF-II. |
Endotoxins | Less than 0.1 EU/ug of rHuIGF-BP4, Insect Cell as determined by LAL method. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
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