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Catalog Number | orb1516414 |
---|---|
Category | Proteins |
Description | Recombinant human IL-7R Alpha / CD127 protein, C-hFc (HEK293) |
Tag | C-hFc |
Concentration | >0.5 mg/ml |
Form/Appearance | Lyophilized |
Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC |
MW | Due to glycosylation, the protein migrates to 55-75 kDa based on Tris-Bis PAGE result. |
Target | Interleukin-7 receptor subunit alpha |
Protein Sequence | 21-236/459 |
Expression System | HEK293 cell |
Biological Activity | Not tested |
Endotoxins | < 1.0 EU/μg as determined by LAL |
Storage | Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles. |
Buffer/Preservatives | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
Alternative names | Interleukin-7 receptor subunit alpha; IL7R; CD 127 Read more... |
Note | For research use only |
Application notes | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Expiration Date | 6 months from date of receipt. |
Human IL-7R alpha /CD127 Protein on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human IL7 at 0.5 µg/ml (100 µl/Well). Dose response curve for Human IL7 R alpha with the EC50 of 67.9 ng/ml determined by ELISA.
The purity of Human IL-7R alpha / CD127 Protein is greater than 95% as determined by SEC-HPLC.