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Catalog Number | orb1921845 |
---|---|
Category | Proteins |
Description | nterleukin (IL)-18, structurally similar to IL-1β, is a member of IL-1 superfamily of cytokines. This cytokine, which is expressed by many human lymphoid and nonlymphoid cells, has an important role in inflammatory processes. The main function of IL-18 is mediated through induction of interferon-γ (IFN-γ) secretion from T helper (Th1) cells. |
Form/Appearance | Lyophilized Powder |
Purity | ≥95% |
MW | 18.2 kDa |
Entrez | 3606 |
UniProt ID | Q14116 |
Source | CHO Cell |
Expression System | Eukaryotic cell |
Biological Origin | Human |
Endotoxins | ≤10 EU/mg |
Storage | Use a manual defrost freezer and avoid repeated freeze - thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -78°C under sterile conditions after reconstitution. |
Alternative names | Interleukin-18; Iboctadekin; Interferon gamma-indu Read more... |
Note | For research use only |
Application notes | Application Notes: For research use only. Minimum Sample Size: 50μg/vial |
Expiration Date | 6 months from date of receipt. |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 19 kDa based on Tris-Bis PAGE result. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 44.3 kDa after removal of the signal peptide.The apparent molecular mass of IL18-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
Greater than 90% as determined by SDS-PAGE. | |
19.7 kDa | |
E.coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |