Recombinant Human Human Trefoil Factor 3 (rHuTFF-3)

• Catalog Number: orb1494968
• Category: Proteins
• Description: The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are secreted in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF-3 is expressed by goblet cells and in the uterus, and has also been shown to express in certain cancers, including colorectal, hepatocellular, and in biliary tumors. TFF3 may be useful as a molecular marker for certain types of cancer, but its role, if any, in tumorigenesis is unknown. TFF3 also promotes airway epithelial cell migration and differentiation.
• Form/Appearance: Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
• Purity: > 95% by SDS-PAGE and HPLC analyses.
• MW: Approximately 6.5 kDa, a single non-glycosylated polypeptide chain containing 59 amino acids.
• Protein Sequence: EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF
• Source: Escherichia coli.
• Biological Activity: Determined by its ability to chemoattract human MCF-7 cells using a concentration range of 1.0-10.0 μg/ml.
• Endotoxins: Less than 1EU/μg of rHuTFF3 as determined by LAL method.
• Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
• Note: For research use only
• Application notes: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.
• Expiration Date: 6 months from date of receipt.