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Catalog Number | orb1516313 |
---|---|
Category | Proteins |
Description | Recombinant human HAVCR2 protein, C-His (HEK293) |
Tag | C-His |
Concentration | >0.5 mg/ml |
Form/Appearance | Lyophilized |
Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC |
MW | Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. |
Target | Hepatitis A virus cellular receptor 2 |
Protein Sequence | 22-200/301 |
Expression System | HEK293 cell |
Biological Activity | Not tested |
Endotoxins | < 1.0 EU/μg as determined by LAL |
Storage | Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles. |
Buffer/Preservatives | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
Alternative names | Hepatitis A virus cellular receptor 2 homolog; Hav Read more... |
Note | For research use only |
Application notes | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Expiration Date | 6 months from date of receipt. |
Immobilized human Tim-3 at 0.5 ug/ml (100 ul/Well), dose response curve for antibody with EC50 of 47.05 ng/ml determined by ELISA.
Recombinant TIM-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
The purity of TIM3 is greater than 95% as determined by SEC-HPLC.
98.00% | |
20.7 kDa (predicted). Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. |
98.00% | |
22.8 kDa (predicted). Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result. |