Recombinant Human FURIN

Catalog Number: orb1100227

Catalog Number	orb1100227
Category	Proteins
Description	Recombinant Human FURIN
Tested applications	ELISA, WB
Reactivity	Human
Tag	N-terminal His-IF2DI Tag
Purity	Greater than 95% by SDS-PAGE gel analyses
MW	48.8 KDa
Formula	Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH₇.4
UniProt ID	P09958
Source	E.Coli
Expression System	108-371
Storage	-20°C for 12 months as lyophilized;2-8°C for 1 month under sterile conditions after reconstitution
Buffer/Preservatives	Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH7.4
Alternative names	Dibasic processing enzyme, Dibasic-processing enzy Read more...
Note	For research use only
Application notes	Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex
Expiration Date	6 months from date of receipt.

Conjugation:	Unconjugated
Purity:	The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
MW:	The protein has a predicted molecular mass of 61.0 kDa after removal of the signal peptide. The apparent molecular mass of FURIN-His is approximately 55-70 kDa due to glycosylation.
Source:	Mammalian

Sizes Available: 10 μg, 50 μg, 100 μg

Conjugation:	Unconjugated
Purity:	95%
MW:	136.3 kDa
Source:	SARS S protein (R667A, K968P, V969P), His Tag (orb750333) is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Pro 1195 (Accession # AAP13567.1 (R667A, K968P, V969P)). The recombinant protein is expressed with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (K968P, V969P) and alanine substitutions (R667A) are introduced to stabilize the trimeric prefusion state of SARS-CoV S protein and abolish the furin cleavage site, respectively.

Sizes Available: 100 μg, 1 mg