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Catalog Number | orb1516453 |
---|---|
Category | Proteins |
Description | Recombinant human FLT3 / Flk-2 protein, C-His-Avi (HEK293) |
Tag | C-His-Avi |
Concentration | >0.5 mg/ml |
Form/Appearance | Lyophilized |
Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC |
MW | Due to glycosylation, the protein migrates to 75-100 kDa based on Tris-Bis PAGE result. |
Target | Receptor-type tyrosine-protein kinase FLT3 |
Protein Sequence | 27-541/993 |
Expression System | HEK293 cell |
Biological Activity | Not tested |
Endotoxins | < 1.0 EU/μg as determined by LAL |
Storage | Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles. |
Buffer/Preservatives | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
Alternative names | Receptor-type tyrosine-protein kinase FLT3; CD135 Read more... |
Note | For research use only |
Application notes | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Expiration Date | 6 months from date of receipt. |
Human FLT3 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Immobilized Human FLT3, His Tag at 0.5 µg/ml (100 µl/Well). Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 85 ng/ml determined by ELISA.
The purity of Human FLT3 is greater than 95% as determined by SEC-HPLC.