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Catalog Number | orb1516468 |
---|---|
Category | Proteins |
Description | Recombinant human CTLA4 protein, C-His (HEK293) |
Tag | C-His |
Concentration | > 0.5 mg/ml |
Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC |
MW | Due to glycosylation, the protein migrates to 28-35 kDa based on Tris-Bis PAGE result. kDa |
Target | Cytotoxic T-lymphocyte protein 4 |
Protein Sequence | 36-161/223 |
Expression System | HEK293 cell |
Endotoxins | < 1.0 EU/μg as determined by LAL |
Storage | Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles. |
Alternative names | CD 152; CD152; CD152 antigen; Celiac disease 3; CE Read more... |
Note | For research use only |
Application notes | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Expiration Date | 6 months from date of receipt. |
Human CTLA4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human CD80, hFc Tag at 0.5 µg/ml (100 µl/Well). Dose response curve for Human CTLA4, His Tag with the EC50 of 82.31 ng/ml determined by ELISA.
Immobilized Human CTLA4, His Tag at 0.5 µg/ml (100 µl/Well). Dose response curve for Human CD86, hFc Tag with the EC50 of 0.16 µg/ml determined by ELISA.
The purity of Human CTLA4 is greater than 95% as determined by SEC-HPLC.
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result. kDa |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result. kDa |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result. kDa |
> 90% as determined by SDS-PAGE | |
25.7 kDa |