Recombinant human CD47 protein, C-His (HEK293)

• Catalog Number: orb1516487
• Category: Proteins
• Description: Recombinant human CD47 protein, C-His (HEK293)
• Tag: C-His
• Concentration: ＞0.5 mg/ml
• Form/Appearance: Lyophilized
• Purity: > 95% as determined by Tris-Bis PAGE
• MW: Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
• Target: Leukocyte surface antigen CD47
• Protein Sequence: 19-139/323
• Expression System: HEK293 cell
• Biological Activity: Not tested
• Endotoxins: < 1.0 EU/μg as determined by LAL
• Storage: Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.
• Alternative names: Antigenic surface determinant protein OA3; CD 47;
• Note: For research use only
• Application notes: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Expiration Date: 6 months from date of receipt.

Human CD47 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Immobilized Human CD47, His Tag at 0.5 µg/ml (100 µl/Well), dose response curve for Human SIRP alpha, Fc Tag with the EC50 of 95.0 ng/ml determined by ELISA.

Immobilized Human CD47, His Tag at 5 µg/ml (100 µl/Well). Dose response curve for Human SIRPa, hFc Tag with the EC50 of 95.0/94.7/93.8 ng/ml determined by ELISA.