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Catalog Number | orb2328768 |
---|---|
Category | Proteins |
Description | Recombinant human CD40 protein, C-His-Avi (HEK293), Biotin conjugated |
Tag | C-His-Avi |
Concentration | >0.5 mg/ml |
Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC |
MW | Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result. |
Target | Tumor necrosis factor receptor superfamily member 5 |
Protein Sequence | 21-193/277 |
Expression System | HEK293 cell |
Biological Activity | Not tested |
Endotoxins | < 1.0 EU/μg as determined by LAL |
Storage | Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles. |
Buffer/Preservatives | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
Alternative names | Tumor necrosis factor receptor superfamily, member Read more... |
Note | For research use only |
Application notes | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Expiration Date | 6 months from date of receipt. |
Biotinylated Recombinant Human CD40 Protein on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Immobilized Biotinylated Human CD40 at 1 ug/ml (100 ul/Well) on streptavidin precoated (0.2 µg/well) plate. Dose response curve for Human CD40L with the EC50 of 15 ng/ml determined by ELISA.
The purity of Biotinylated Recombinant Human CD40 Protein is greater than 95% as determined by SEC-HPLC.