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Catalog Number | orb1906267 |
---|---|
Category | Proteins |
Description | Recombinant Human Amphiregulin |
Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
Purity | > 95 % by SDS-PAGE and HPLC analyses. |
MW | Approximately 11.3 kDa, a single non-glycosylated polypeptide chain containing 98 amino acid residues. |
UniProt ID | P15514 |
Protein Sequence | SVRVEQVVKP PQNKTESENT SDKPKRKKKG GKNGKNRRNR KKKNPCNAEF QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMKTHS MIDSSLSK |
Source | Escherichia coli |
Expression System | Escherichia coli |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is between 5-10 ng/ml. |
Endotoxins | Less than 1 EU/ug of rHuAmphiregulin as determined by LAL method. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Alternative names | AR, AREG, Colorectum Cell-derived Growth Factor, C Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
HPLC, SDS-PAGE | |
Unconjugated | |
> 96% by SDS-PAGE and HPLC analyses | |
9.0 kDa |
≥90% as determined by SDS-PAGE | |
This protein contains the human AREG(Ser101-Lys198) was fused without Tag and expressed in E. coli. |
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