Recombinant HLA-Pan (MHC II) Antibody

• Catalog Number: orb608003
• Category: Antibodies
• Description: Rabbit monoclonal antibody to HLA-Pan (MHC II)
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: HLA-Pan/2967R
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Non-T, non-B human acute lymphoblastic leukemia REH6 cell line
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: ELISA (For coating use Ab at 2-5ug/ml, order Ab without BSA); Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 33-35kDa
• Target: HLA-DPB1
• Entrez: 3122, 3117, 3115
• UniProt ID: P01920, P01903, P04440, P01909, P01908
• Expression System: HEK293 cells
• Storage: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: HLA-DPB1; HLA-DQA1; HLA-DQB1; HLA-DRA; HLA-DRB1; H
• Note: For research use only
• Application notes: Positive Control: Ramos or Raji cells. Human tonsil or lymph node
• Expiration Date: 12 months from date of receipt.