Recombinant HCC-1/CCL14 (72aa), Human

• Catalog Number: orb1494848
• Category: Proteins
• Description: HCC-1/CCL14 is a member of the chemokine family, which are small chemotactic proteins that regulate cell migration under inflammatory and steady state conditions. HCC-1 is expressed in epithelial and decidual cells and is unique among chemokines due to its high abundance in normal human plasma. HCC-1 can bind to chemokine receptors CCR1 and CCR5, however full length HCC-1 is a weak agonist of CCR1 and only becomes potent after removal of its eight N-terminal residues. Chemokine decoy receptor D6 can bind HCC-1 and promote its degradation as a means to regulate its level in vivo. Functionally HCC-1 promotes trophoblast migration by regulating extracellular matrix components as well as specific adhesion molecules.Recombinant human Hemofiltrate CC Chemokine-1 (72 a.a.) (HCC-1)/CCL14 (rhHCC-1) produced in E.coli is a single non-glycosylated polypeptide chain containing 72 amino acids. A fully biologically active molecule, rhHCC-1 has a molecular mass of 8.4kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• MW: 8.4 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: TES SSRGPYHPSE CCFTYTTYKI PRQRIMDYYE TNSQCSKPGI VFITKRGHSV CTNPSDKWVQ DYIKDMKEN
• Source: Escherichia coli.
• Biological Activity: ED50 40 units/mg.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant human Hemofiltrate CC Chemokine-1 (72 a.a.) (HCC-1)/CCL14 (rhHCC-1) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhHCC-1 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Alternative names: NCC-2, SCYA14
• Note: For research use only
• Application notes: Reconstituted in ddH2O at 100 μg/mL.
• Expiration Date: 6 months from date of receipt.