Recombinant HB-EGF, Human

• Catalog Number: orb1494849
• Category: Proteins
• Description: Proheparin-binding EGF-like growth factor (HB-EGF), also known as DTR, DTS and HEGFL, is a member of the EGF family of mitogens. It is expressed in macrophages, monocytes, endothelial cells and muscle cells. HB-EGF signals through the EGF receptor to stimulate the proliferation of smooth muscle cells, epithelial cells and keratinocytes. Compared to EGF, HB-EGF binds to the EGF receptor with a higher affinity and has been shown to bemore mitogenic, likely due to its ability to bind to heparin and heparin sulfate proteoglycans. HB-EGF has also been reported to act as a diphtheria toxin receptor, mediating endocytosis of the bound toxin. Heparin-binding EGF-like growth factor has been shown to interact with NRD1, Zinc finger and BTB domain-containing protein 16 and BAG1.Recombinant Human HB-EGF produced in E.coli cells is a polypeptide chain containing 86 amino acids. A fully biologically active molecule, rhHB-EGF has a molecular mass of 14 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• MW: 14 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: GD LQEADLDLLR VTLSSKPQAL ATPNKEEHGK RKKKGKGLGK KRDPCLRKYK DFCIHGECKY VKELRAPSCI CHPGYHGERC HGLSL
• Source: Escherichia coli.
• Biological Activity: ED50 < 0.75ng/ml, measured in a cell proliferation assay using 3T3 cells.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant Human Proheparin-binding EGF-like Growth Factor (HB-EGF), remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, human HB-EGF should be stable up to 1 week at 4°C or up to 3 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Alternative names: DTR, DTS, HEGFL
• Note: For research use only
• Application notes: Reconstituted in ddH2O or PBS at 100 μg/ml.
• Expiration Date: 6 months from date of receipt.