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Catalog Number | orb1463560 |
---|---|
Category | Antibodies |
Description | Recombinant GST antibody |
Clonality | Recombinant |
Clone Number | GST13 |
Tested applications | DOT, WB |
Concentration | 0.5 mg/ml |
Dilution range | WB: 1:200 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-GST (VHH) Antibody is a recombinant antibody. The clone was isolated from a library prepared from a hyper-immunized llama host and purified by affinity chromatography from bacterial culture. Expect reactivity against GST-labeled probes and proteins. |
Conjugation | Unconjugated |
Source | E. coli |
Biological Origin | E. coli |
Storage | Store at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | recombinant anti-GST antibody, Glutathione-S-Trans Read more... |
Note | For research use only |
Application notes | Anti-GST is a his-tagged monoclonal recombinant antibody designed to detect Glutathione-S-Transferase and its conjugates. This antibody has been tested by western blot and dot blot and is intended for use in immunological assays including western blotting, immunofluorescence and fluorescence activated cell sorting (FACS). Fluorochrome conjugated anti-GST can be used for detection of sensitive non-radioactive in situ hybridization probes. The antibody can be labeled with dyes, enzymes or fluorescence, directly or secondarily, for visualization and detection of GST-conjugated molecules by immunoblotting or immunofluorescence. Secondary detection can be achieved using conjugated anti-His tag or anti-VHH antibodies. Optimal titers for applications should be confirmed by the end user. |
Expiration Date | 12 months from date of receipt. |
Dot Blot of Anti-GST (VHH) Single Domain Antibody. Row A: Recombinant GST protein (p/n orb345956). Amount Dotted: (1) 200, (2) 100, (3) 50, (4) 25, (5) 12.5 ng. Primary Antibody: Anti-GST (VhH) 1.0 µg/mL for 1.5 hr at RT. Secondary Antibody: Mouse Anti-6X His Tag Peroxidase (p/n orb344539) at 1:500 for 30 mins at RT. Block: BlockOut Buffer (p/n orb348644).
Western Blot of Anti-GST Single Domain Antibody. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: Recombinant GST (p/n orb345956) (0.2 µg) [+]. Lane 3: HEK293T WCL / rec. GST (p/n orb345956) (10 µg/0.2 µg) [+]. Lane 4: HEK293T Whole Cell Lysate (10 µg) [-]. Lane 5: E.coli Host Cell Protein (HCP) Control Protein (p/n orb342262) (10 µg) [-]. Primary Antibody: Anti-GST (VhH) at 5.0 µg/mL overnight at 2-8°C. Secondary Antibody: Mouse Anti-6X His Tag Peroxidase (p/n orb344539) at 1:3000 for 30 mins at RT. Block: BlockOut Buffer (p/n orb348644). Exposure: 60 sec. Predicted MW: ~25.5 kDa. Observed MW: ~25.5 kDa. Unspecific Bands: ~70 kDa minor background due to unspecific cross-reactivity of anti-6X His ab with unknown proteins of HEK293T lysate.
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