Recombinant GFAP (Astrocyte & Neural Stem Cell Marker) Antibody

• Catalog Number: orb2309293
• Category: Antibodies
• Description: Recombinant GFAP (Astrocyte & Neural Stem Cell Marker) Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: rGFAP/9150
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: IgG2b
• Immunogen: Recombinant fragment (around aa100-300) of human GFAP protein (exact sequence is proprietary)
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: Immunohistochemistry (Formalin-fixed) (0.25-2ug/ml for 30 minutes at RT);(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: ~50kDa
• Target: GFAP
• Entrez: 2670
• UniProt ID: P14136
• Expression System: CHO cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Astrocyte or Intermediate Filament Protein, Glial
• Note: For research use only
• Application notes: Positive Control: T98G cells. Human brain or astrocytoma
• Expiration Date: 12 months from date of receipt.