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Catalog Number | orb1494741 |
---|---|
Category | Proteins |
Description | Granulocyte Colony-Stimulating Factor (G-CSF), also known as CSF-3 and MGI-1G, is a cytokine and hormone belonging to the IL-6 superfamily. It is expressed by monocytes, macrophages, endothelial cells, fibroblasts and bone marrow stroma. G-CSF stimulates the bone marrow to produce granulocytes and stem cells, and specifically stimulates the proliferation and differentiation of the neutrophilic granulocyte lineage. G-CSF has been used to stimulate white blood cell production after chemotherapy. It has also been used to boost the number of hematopoietic stem cells after bone marrow transplantation. |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
MW | 22-24 kDa, observed by reducing SDS-PAGE. |
Protein Sequence | VPLVTVSALPPSLPLPRSFLLKSLEQVRKIQASGSVLLEQLCATYKLCHPEELVLLGHSLGIPKASLSGCSSQALQQTQC LSQLHSGLCLYQGLLQALSGISPALAPTLDLLQLDVANFATTIWQQMENLGVAPTVQPTQSAMPAFTSAFQRRAGGVLAI SYLQGFLETARLALHHLA |
Source | CHO |
Biological Activity | ED50 < 0.02ng/ml, measured in a cell proliferation assay using NFS-60 cells. |
Endotoxins | < 0.2 EU/μg, determined by LAL method. |
Storage | Lyophilized recombinant murine G-CSF remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, murine G-CSF should be stable up to 1 week at 4°C or up to 2 months at -20°C. |
Buffer/Preservatives | Lyophilized after extensive dialysis against PBS. |
Alternative names | Granulocyte Colony-Stimulating Factor, CSF-3, CSF3 Read more... |
Note | For research use only |
Application notes | Reconstituted in ddH2O or PBS at 100 μg/ml. |
Expiration Date | 6 months from date of receipt. |
> 98% as determined by SDSPAGE and HPLC. | |
18.9 kDa | |
E.Coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
ELISA | |
> 98% as determined by SDS-PAGE. Ni-NTA chromatography. | |
Escherichia coli |
≥90% as determined by SDS-PAGE | |
This protein contains the mouse Csf3r(Val31-Ala208) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
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