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Catalog Number | orb1494864 |
---|---|
Category | Proteins |
Description | Fibroblast Growth Factor-basic (FGF-basic), also known as HBGF-2, is a non-glycosylated heparin-binding growth factor that belongs to the FGF family. FGF-basic is present in basement membranes and in the subendothelial extracellular matrix of blood vessels. FGF-basic signals through FGFR1, 2, 3 and 4 that plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration.Recombinant mouse Fibroblast Growth Factor-basic (rmFGF-basic) produced in E.coli is a single non-glycosylated polypeptide chain containing 146 amino acids. A fully biologically active molecule, rmFGF-basic has a molecular mass of 16.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript. |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
MW | 16.4 kDa, observed by reducing SDS-PAGE. |
Protein Sequence | MPALPEDGGA AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS |
Source | Escherichia coli. |
Biological Activity | ED50 2.0× 10ˆ6 units/mg. |
Endotoxins | < 0.2 EU/μg, determined by LAL method. |
Storage | Lyophilized recombinant mouse Fibroblast Growth Factor-basic (rmFGF-basic) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmFGF-basic should be stable up to 2 weeks at 4°C or up to 3 months at -20°C. |
Buffer/Preservatives | Lyophilized after extensive dialysis against PBS. |
Alternative names | Fibroblast Growth Factor-basic, FGF-2, FGF2, HBGF- Read more... |
Note | For research use only |
Application notes | Reconstituted in ddH2O at 100 μg/ml. |
Expiration Date | 6 months from date of receipt. |
> 95% by SDS-PAGE. | |
KMP1631, Recombinant Mouse FGF-2/FGF basic/bFGF Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Ser154) of mouse FGF-2/FGF basic/bFGF (Accession #P15655). |
≥90% as determined by SDS-PAGE | |
This protein contains the mouse Fgf2(Met1-Ser154) was fused without Tag and expressed in E. coli. |
15.8 kDa, observed by reducing SDS-PAGE. | |
Escherichia coli. |
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