Recombinant FGF-8, Human

• Catalog Number: orb1494877
• Category: Proteins
• Description: Fibroblast Growth Factor-8 (FGF-8) is a heparin-binding growth factor of the FGF family. There are 4 known forms of FGF8 produced by alternative splicing: FGF8a, FGF-8b, FGF-8e and FGF-8f. The human and mouse FGF8b are identical of aa sequences. FGF-8 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. FGF-8 is required for normal brain, eye, ear and limb development during embryogenesis. It is also required for normal development of the gonadotropin- releasing hormone (GnRH) neuronal system.Recombinant human Fibroblast Growth Factor-8 (rhFGF-8) produced in E.coli is a single non-glycosylated polypeptide chain containing 194 amino acids. A fully biologically active molecule, rhFGF-8 has a molecular mass of 22.5kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• MW: 22.5kDa, observed by reducing SDS-PAGE.
• Protein Sequence: MQVTVQSSPN FTQHVREQSL VTDQLSRRLI RTYQLYSRTS GKHVQVLANK RINAMAEDGD PFAKLIVETD TFGSRVRVRG AETGLYICMN KKGKLIAKSN GKGKDCVFTE IVLENNYTAL QNAKYEGWYM AFTRKGRPRK GSKTRQHQRE VHFMKRLPRG HHTTEQSLRF EFLNYPPFTR SLRGSQRTWA PEPR
• Source: Escherichia coli.
• Biological Activity: ED50 2.0× 10ˆ5 units/mg.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant human Fibroblast Growth Factor-8 (rhFGF-8) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhFGF-8 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Alternative names: FGF8; FGF-8b, AIGF, HBGF
• Note: For research use only
• Application notes: Reconstituted in ddH2O at 100 μg/ml.
• Expiration Date: 6 months from date of receipt.