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Catalog Number | orb1494878 |
---|---|
Category | Proteins |
Description | Fibroblast Growth Factor-6 (FGF-6) is a cytokine belonging to the heparin-binding FGF family, and is structurally related to other members of FGF family, particularly FGF-4. In vivo, FGF-6 exhibits an expression profile predominantly restricted tothe myogenic lineage, and it preferentially binds to two of the FGF receptors: FGFR1 and FGFR4. FGF-6 functions in muscle regeneration, myoblast proliferation and migration, and muscle differentiation in a dose-dependent manner. In vivo high concentration of recombinant FGF-6 up-regulates and down-regulates FGFR1 and FGFR4, respectively, as FGFR1 promotes the proliferation while FGFR4 promotes the differentiation in the muscle. Besides its dual function in muscle regeneration, FGF-6 may act as a regulator of bone metabolism as well.Recombinant human Fibroblast Growth Factor-6 (rhFGF-6) produced in E.coli is a single non-glycosylated polypeptide chain containing 169 amino acids. A fully biologically active molecule, rhFGF-6 has a molecular mass of 18.8 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript. |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
MW | 18.8 kDa, observed by reducing SDS-PAGE. |
Protein Sequence | MGTRANNTL LDSRGWGTLL SRSRAGLAGE IAGVNWESGY LVGIKRQRRL YCNVGIGFHL QVLPDGRISG THEENPYSLL EISTVERGVV SLFGVRSALF VAMNSKGRLY ATPSFQEECK FRETLLPNNY NAYESDLYQG TYIALSKYGR VKRGSKVSPI MTVTHFLPRI |
Source | Escherichia coli. |
Biological Activity | ED50 4× 10ˆ5 units/mg. |
Endotoxins | < 0.2 EU/μg, determined by LAL method. |
Storage | Lyophilized recombinant human Fibroblast Growth Factor-6 (rhFGF-6) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhFGF-6 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. |
Buffer/Preservatives | Lyophilized after extensive dialysis against PBS. |
Alternative names | FGF6; Fibroblast Growth Factor-6, HBGF-6, HST-2 Read more... |
Note | For research use only |
Application notes | Reconstituted in ddH2O at 100 μg/mL. |
Expiration Date | 6 months from date of receipt. |
ELISA | |
> 98% as determined by SDS-PAGE. Ni-NTA chromatography. | |
Escherichia coli |
≥90% as determined by SDS-PAGE | |
This protein contains the human FGF6(Gly41-Ile208) was fused without Tag and expressed in E. coli. |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
18.4 kDa | |
E.Coli |
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