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Catalog Number | orb1494879 |
---|---|
Category | Proteins |
Description | Fibroblast Growth Factor-4 (FGF-4) also known as K-FGF is a heparin-binding growth factor of the FGF family.It was identified by its oncogenic transforming activity. FGF-4 and FGF-3 are located closely on chromosome 11. FGF-4 and its receptors (FGF R1c, 2c, 3c and 4) play an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. FGF-4 is required for normal limb and cardiac valve development during embryogenesis.Recombinant human Fibroblast Growth Factor-4 (rhFGF-4) produced in E.coli is a single non-glycosylated polypeptide chain containing 177 amino acids. A fully biologically active molecule obtained by proprietary chromatographic techniques at GenScript, rhFGF-4 has a molecular mass of 19.4kDa as analyzed by reducing SDS-PAGE. |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
MW | 19.4kDa, observed by reducing SDS-PAGE. |
Protein Sequence | MAPTAPNGT LEAELERRWE SLVALSLARL PVAAQPKEAA VQSGAGDYLL GIKRLRRLYC NVGIGFHLQA LPDGRIGGAH ADTRDSLLEL SPVERGVVSI FGVASRFFVA MSSKGKLYGS PFFTDECTFK EILLPNNYNA YESYKYPGMF IALSKNGKTK KGNRVSPTMK VTHFLPRL |
Source | Escherichia coli. |
Biological Activity | ED502.0× 10ˆ6 units/mg. |
Endotoxins | < 0.2 EU/μg, determined by LAL method. |
Storage | Lyophilized recombinant human Fibroblast Growth Factor-4 (rhFGF-4) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhFGF-4 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C. |
Buffer/Preservatives | Lyophilized after extensive dialysis against 50mM HEPES, 750mM NaCl, pH 7.5. |
Alternative names | FGF4; HBGF-4; HST; HST-1; HSTF1; K-FGF; KFGF. Read more... |
Note | For research use only |
Application notes | Reconstituted in ddH2O at 100 μg/ml. |
Expiration Date | 6 months from date of receipt. |
> 96% as determined by SDSPAGE and HPLC. | |
19.8 kDa | |
E.Coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
Unconjugated | |
Greater than 95% as determined by reducing SDS-PAGE. | |
15.1 KDa | |
E. Coli |
Greater than 95% as determined by SDS-PAGE. | |
16.9 kDa | |
E.coli |
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