Recombinant FGF-21, Human

• Catalog Number: orb1494881
• Category: Proteins
• Description: Fibroblast Growth Factor-21 (FGF-21) is a metabolic cytokine belonging to the heparin-binding FGF family. Along with FGF-19/15 and FGF-23, FGF-21 is categorized as a member of the atypical FGF subfamily, as it must be complexed to the Klotho co-receptor in order to bind to the FGF receptors and activate the downstream signaling pathway. In vivo FGF-21 is expressed in liver, pancreas, adipose tissue, and skeletal muscle, and it plays a central role in the energy metabolism. The expression of FGF-21 is stimulated by free fatty acids and insulin resistant states and is correlated with whole-body insulin resistance. FGF-21 activates glucose uptake in adipocytes and increases insulin sensitivity, implicating it as a novel target with potential anti-diabetic properties.Recombinant human Fibroblast Growth Factor-21 (rhFGF-21) produced in E.coli is a single non-glycosylated polypeptide chain containing 182 amino acids. A fully biologically active molecule, rhFGF-21 has a molecular mass of 19.5 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• MW: 19.5 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: GH PIPDSSPLLQ FGGQVRQRYL YTDDAQQTEA HLEIREDGTV GGAADQSPES LLQLKALKPG VIQILGVKTS RFLCQRPDGA LYGSLHFDPE ACSFRELLLE DGYNVYQSEA HGLPLHLPGN KSPHRDPAPR GPARFLPLPG LPPALPEPPG ILAPQPPDVG SSDPLSMVGP SQGRSPSYAS
• Source: Escherichia coli.
• Biological Activity: ED50 2× 10ˆ3 units/mg.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant human Fibroblast Growth Factor-21 (rhFGF-21) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhFGF-21 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Alternative names: FGF21, fibroblast growth factor-21, FGFL
• Note: For research use only
• Application notes: Reconstituted in ddH2O at 100 μg/mL.
• Expiration Date: 6 months from date of receipt.