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Catalog Number | orb1494887 |
---|---|
Category | Proteins |
Description | Fibroblast Growth Factor-10 (FGF-10) is a mitogen mainly produced by mesenchymal stem cells in the lung. FGF-10 belongs to the heparin binding FGF family, and is also known as Keratinocyte Growth Factor-2 (KGF-2). It shares homology with KGF and receptor binding to FGFR2-IIIb. However, while KGF induces proliferation and differentiation of various epithelial cells, FGF-10 promotes budding and branching morphogenesis during the multi-organ development via mesenchymal-epithelial cell interactions. FGF-10 is critical for lung and limb development, and is regulated by Shh during early development. Recombinant Human FGF-10 produced in E.coli is a single, non-glycosylated polypeptide chain containing 169 amino acids. A fully biologically active molecule, rhFGF-10 has a molecular mass of 19.3 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript. |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
MW | 19.3 kDa, observed by reducing SDS-PAGE. |
Protein Sequence | LGQDMVSPEA TNSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGK VS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIE EN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS |
Source | Escherichia coli. |
Biological Activity | ED50 5.0 × 10ˆ4 units/mg. |
Endotoxins | < 0.2 EU/μg, determined by LAL method. |
Storage | Lyophilized recombinant Human FGF-10 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Human FGF-10 should be stable up to 1 week at 4°C or up to 3 months at -20°C. |
Buffer/Preservatives | Lyophilized after extensive dialysis against PBS. |
Alternative names | Fibroblast Growth Factor-10, FGFA, Keratinocyte gr Read more... |
Note | For research use only |
Application notes | Reconstituted in ddH2O at 100 μg/ml. |
Expiration Date | 6 months from date of receipt. |
> 97 % by SDS-PAGE and HPLC analyses. | |
Approximately 19.1 kDa, a single, non-glycosylated polypeptide chain containing 169 amino acids. | |
Escherichia coli |
> 97% as determined by SDS-PAGE and HPLC. | |
19.1 kDa | |
E.Coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
HPLC, SDS-PAGE | |
Unconjugated | |
> 96% by SDS-PAGE and HPLC analyses | |
20.0 kDa |
Unconjugated | |
Greater than 95% as determined by reducing SDS-PAGE. | |
19.5 KDa | |
E. Coli |
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