Recombinant Enterokinase, Light Chain, Porcine

• Catalog Number: orb1494616
• Category: Proteins
• Description: GenScript Enterokinase is a highly purified recombinant porcine enterokinase. The enzyme has been extensively purified and tested to ensure that there are no other contaminating proteases. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not cleave, however, if this lysine is followed by a proline. Enterokinase can remove fusion tags is located in the N-terminal section of proteins, useful for removing unwanted tags.
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• MW: Theoretical MW: 21,880 DaThe apparent MW on SDS-PAGE: about 40,000 Da
• Protein Sequence: 0
• Source: P. pastoris
• Biological Activity: One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95% completion at 22°C in a buffer containing 25 mM Tris-HCl, pH 8.0.
• Storage: Store at -20°C after delivery.
• Buffer/Preservatives: GenScript enterokinase, formulated using GenScript's proprietary technology, can be shipped at room temperature. It will remain stable at 37°C for one week without losing any activity.
• Alternative names: Enteropeptidase, ENTK, PRSS7
• Note: For research use only
• Application notes: Reconstituted in ddH2O or PBS at 100 μg/ml.
• Expiration Date: 6 months from date of receipt.