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Catalog Number | orb1494618 |
---|---|
Category | Proteins |
Description | Endostatin is a cleaved product of the carboxyl-terminal domain of collagen XVIII. It functions as an anti-angiogenic cytokine that is expressed in various organs especially with high levels in liver, lung, and kidney. Endostatin inhibits angiogenesis by blocking the pro-angiogenic activities of VEGF and FGF-basic. Recombinant Endostatin expressed in yeast, whichcauses G1 arrest of endothelial cells. Endostatin treatment results in apoptosis of HUVE and HMVE cells. Recombinant human Endostatin is a 20.2 kDa protein consisting of 184 amino acid residues. |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
MW | ~20.2 kDa, observed by reducing SDS-PAGE. |
Protein Sequence | AH SHRDFQPVLH LVALNSPLSG GMRGIRGADF QCFQQARAVG LAGTFRAFLS SRLQDLYSIV RRADRAAVPI VNLKDELLFP SWEALFSGSE GPLKPGARIF SFDGKDVLRH PTWPQKSVWH GSDPNGRRLT ESYCETWRTE APSATGQASS LLGGRLLGQS AASCHHAYIV LCIENSFMTA SK |
Source | P. pastoris |
Biological Activity | Determined by Endothelial Cell Tube Formation. The cells treated with 10μg/ml Endostatin showed significant anti-angiogenesis effect. |
Endotoxins | Endotoxin level is less than 0.1 ng per μg (1EU/μg) of Endostatin. |
Storage | The lyophilized Endostatin is stable for a few weeks at room temperature, but best stored at -20°C. For the first use, reconstitute in water, aliquot and store at -20°C to avoid multiple freeze-thaws. |
Buffer/Preservatives | The sterile filtered solution was lyophilized with 17 mM citric-phosphate buffer, pH 6.2. |
Alternative names | Endostatin Read more... |
Note | For research use only |
Application notes | Reconstituted in ddH2O or PBS at 100 μg/ml. |
Expiration Date | 6 months from date of receipt. |
> 96 % by SDS-PAGE and HPLC analyses. | |
Approximately 20.1 kDa, a single non-glycosylated polypeptide chain containing 183 amino acids (C-terminal fragment of Collagen alpha-1(XVIII) chain). | |
Escherichia coli |
Greater than 96% as determined by SDS-PAGE and HPLC. | |
19.8 kDa | |
E.Coli |
Greater than 90% as determined by SDS-PAGE. | |
46.3 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
26.5 kDa | |
E.coli |
SDS-PAGE | |
Unconjugated | |
> 96% by SDS-PAGE and HPLC analyses. | |
19.8 kDa | |
E. coli |
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