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Catalog Number | orb1494895 |
---|---|
Category | Proteins |
Description | Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Recombinant human Epidermal Growth Factor (EGF) is a 6,200 Da protein containing 53 amino acid residues. |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
MW | 6.0 kDa+/-10% determined by reduced SDS-PAGE |
Protein Sequence | MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR |
Source | Escherichia coli. |
Biological Activity | The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less then 2 ng/ml, corresponding to a specific activity of 5.0×10ˆ5 IU/ mg. |
Endotoxins | Less than 0.1ng/μg (1 EU/μg) of recombinant human Epidermal Growth Factor (EGF) as determined by LAL test. |
Storage | Lyophilized recombinant Human Epidermal Growth Factor (rhEGF) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhEGF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C. |
Buffer/Preservatives | Recombinant human Epidermal Growth Factor (EGF) was lyophilized after extensive dialysis against 10mM Phosphate buffer, pH 7.0, 200mM NaCl buffer. |
Alternative names | Epidermal Growth Factor Read more... |
Note | For research use only |
Application notes | It is recommended to reconstitute the lyophilized recombinant human Epidermal Growth Factor (EGF) in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
Expiration Date | 6 months from date of receipt. |
> 97 % by SDS-PAGE and HPLC analyses. | |
Approximately 7.4 kDa, a single non-glycosylated polypeptide chain containing 65 amino acids. | |
Escherichia coli |
> 96 % by SDS-PAGE and HPLC analyses. | |
Approximately 7.0 kDa, a single non-glycosylated polypeptide chain containing 61 amino acids. | |
Escherichia coli |
> 97% by SDS-PAGE and HPLC analyses. | |
Approximately 9.7 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids. | |
Escherichia coli |
> 97 % by SDS-PAGE and HPLC analyses. | |
Approximately 7.5 kDa, a single non-glycosylated polypeptide chain containing 65 amino acids. | |
Escherichia coli |
> 97% as determined by SDS-PAGE and HPLC. | |
9.7 kDa | |
E.Coli |
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