Recombinant Cytokeratin 8/18 Antibody

• Catalog Number: orb607982
• Category: Antibodies
• Description: Mouse monoclonal antibody to Cytokeratin 8/18
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: rKRT8.18/1346
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Recombinant full-length human KRT8 protein & Recombinant full-length human KRT18 protein
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 52.5kDa (CK8); 45kDa (CK18)
• Target: KRT8
• Entrez: 3856, 3875
• UniProt ID: P05787
• Expression System: HEK293 cells
• Storage: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Buffer/Preservatives: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: CARD2; CK8; CYK8; CYKER; Cytokeratin Endo A; DreK8
• Note: For research use only
• Application notes: Positive Control: MCF-7 or A431 cells. Skin, colon, lung or breast carcinoma.
• Expiration Date: 12 months from date of receipt.