Recombinant Cytokeratin 8/18 Antibody

• Catalog Number: orb1410015
• Category: Antibodies
• Description: Rabbit monoclonal antibody to Cytokeratin 8/18
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: KRT8.18/6579R
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant full-length human CK8 and CK8 proteins
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 52.5kDa (CK8); 45kDa (CK18)
• Target: KRT18|KRT8
• Entrez: 3856, 18, 8, 3875
• UniProt ID: P05787, P05783
• Source: Expressed in HEK293 cells
• Expression System: CHO cells
• Storage: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: CARD2; CK8; CYK8; CYKER; Cytokeratin Endo A; DreK8
• Note: For research use only
• Application notes: Positive Control: MCF-7 or A431 cells. Human skin, colon, lung or breast carcinoma.
• Expiration Date: 12 months from date of receipt.