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Catalog Number | orb1516501 |
---|---|
Category | Proteins |
Description | Recombinant Cyn. monkey CD3E & CD3G protein, C-hFc (HEK293) |
Tag | C-hFc |
Concentration | > 0.5 mg/ml |
Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC |
MW | Due to glycosylation,the protein migrates to 44-46 & 46-48 kDa based on Tris-Bis PAGE result. kDa |
Target | T-cell surface glycoprotein CD3 epsilon chain |
Protein Sequence | 20-198/198 |
Expression System | HEK293 cell |
Endotoxins | < 1.0 EU/μg as determined by LAL |
Storage | Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles. |
Alternative names | CD3; CD3D; CD3-DELTA; T3D; CD3G;Recombinant Cyn. m Read more... |
Note | For research use only |
Application notes | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Expiration Date | 6 months from date of receipt. |
Immobilized Cyno CD3 Epsilon & CD3 Gamma, hFc Tag at 0.5 µg/ml (100 µl/Well). Dose response curve for Biotinylated SP34, hFc Tag with EC50 of 14.0 ng/ml /14.6 ng/ml /13.6 ng/ml determined by ELISA.
Immobilized Cyno CD3 Epsilon & CD3 Gamma, hFc Tag at 0.5 µg/ml (100 µl/Well). Dose response curve for Biotinylated SP34, hFc Tag with EC50 of 14.0 ng/ml determined by ELISA.
The purity of Cynomolgus CD3E&CD3G / CD3 Epsilon&CD3 Gamma was greater than 95% as determined by SEC-HPLC.
ynomolgus CD3E&CD3G / CD3 Epsilon&CD3 Gamma on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.