Recombinant CNTF, Mouse

• Catalog Number: orb1494900
• Category: Proteins
• Description: Ciliary Neurotrophic Factor (CNTF) is a polypeptide hormone which acts within the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. CNTF is a potent survival factor for neurons and oligodendrocytes and may play a role in reducing tissue damage during increased inflammation. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, however this phenotype is not causally related to neurologic disease. Recombinant Mouse CNTF produced in E.coli is a single, non-glycosylated polypeptide chain of 197 amino acids and a molecular mass of 22.6 kDa. It has been purified by chromatographic techniques.
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• MW: 22.6 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: AFAEQSPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNISLD SVDGVPVAS T DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LTLQVSAFA Y QLEELMALLE QKVPEKEADG MPVTIGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVIS S HHMGISAHES HYGAKQM
• Source: Escherichia coli.
• Biological Activity: ED50 < 30ng/ml, measured by its ability to induce alkaline phosphatase production byTF-1 Cells.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant Mouse CNTF remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Mouse CNTF should be stable up to 1 week at 4°C or up to 3 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS
• Alternative names: Ciliary Neurotrophic Factor
• Note: For research use only
• Application notes: Reconstituted in ddH2O at 100 μg/ml.
• Expiration Date: 6 months from date of receipt.