Recombinant Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) Antibody

• Catalog Number: orb750916
• Category: Antibodies
• Description: Mouse monoclonal antibody to CALD1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: rCALD1/820
• Tested applications: WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Recombinant full-length human CALD1 protein
• Concentration: 200 μg/ml
• Dilution range: Western Blot (1-2ug/ml); ,Immunohistology (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 120-150kDa
• Target: CALD1
• Entrez: 800
• UniProt ID: Q05682
• Expression System: HEK293 cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: Anti-CAD antibody, anti-CALD1 antibody, anti-Calde
• Note: For research use only
• Application notes: Positive Control: Ovary, Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma
• Expiration Date: 12 months from date of receipt.