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Catalog Number | orb1494656 |
---|---|
Category | Proteins |
Description | Amphiregulin is a member of the EGF family of cytokines, which comprises at least ten proteins including EGF, TGF-α, HB-EGF, Epiregulin, Tomoregulin, Neuregulins and the various heregulins. Through the EGF/TGF-α receptor, it stimulates growth of keratinocytes, epithelial cells and some fibroblasts. Amphiregulin also inhibits the growth of certain carcinoma cell lines. Synthesized as a transmembrane protein, Amphiregulin’s extracellular domain is proteolytically processed to release the mature protein. |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
MW | 15~20 kDa, observed by reducing SDS-PAGE. |
Protein Sequence | SVRVEQVVKPPQNKTESENTSDKPKRKKKGGKNGKNRRNRKKKNPCNAEFQNFCIHGECKYIEHLEAVTCKCQQEYFGER CGEKSMKTHSMIDSSLSK |
Source | HEK 293 |
Biological Activity | ED50 < 0.2 ng/ml, measured in a cell proliferation assay using 3T3 cells. |
Endotoxins | < 0.2 EU/μg, determined by LAL method. |
Storage | Lyophilized recombinant Human Amphiregulin remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Human Amphiregulin should be stable up to 1 week at 4°C or up to 2 months at -20°C. |
Buffer/Preservatives | Lyophilized after extensive dialysis against PBS. |
Alternative names | AR, AREG, Colorectum cell-derived growth factor (C Read more... |
Note | For research use only |
Application notes | Reconstituted in ddH2O or PBS at 100 μg/ml. |
Expiration Date | 6 months from date of receipt. |
> 95 % by SDS-PAGE and HPLC analyses. | |
Approximately 11.3 kDa, a single non-glycosylated polypeptide chain containing 98 amino acid residues. | |
Escherichia coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
HPLC, SDS-PAGE | |
Unconjugated | |
> 96% by SDS-PAGE and HPLC analyses | |
9.0 kDa |
≥90% as determined by SDS-PAGE | |
This protein contains the human AREG(Ser101-Lys198) was fused without Tag and expressed in E. coli. |
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