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Catalog Number | orb1494910 |
---|---|
Category | Proteins |
Description | Adiponectin is a hormone mainly produced by adipocytes. Adiponectin forms a homotrimer and exists as higher order multimers in vivo. The receptors of Adiponectin are seven-transmembrane G protein coupled receptors: Receptor 1 is expressed in skeletal muscle and Receptor 2 in liver. Adiponectin receives a lot of attention because of its anti-diabetic, anti-atherosclerotic, and anti-inflammatory properties. Adiponectin increases the expression of molecules involved in fatty acid transport, combustion of fatty acid, and energy dissipation, and increases insulin sensitivity of the body. Decreased levels of Adiponectin are associated with hypertension, cardiovascular diseases, and metabolic syndromes. Therefore, Adiponectin has promising potential as a pharmacological agent. Recombinant human Adiponectin (rhAdiponectin) produced in E.coli is a single non-glycosylated polypeptide chain containing 227 amino acids. A fully biologically active molecule, rhAdiponectin has a molecular mass of 24.7 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript. |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
MW | 24.7 kDa, observed by reducing SDS-PAGE. |
Protein Sequence | METTTQGPG VLLPLPKGAC TGWMAGIPGH PGHNGAPGRD GRDGTPGEKG EKGDPGLIGP KGDIGETGVP GAEGPRGFPG IQGRKGEPGE GAYVYRSAFS VGLETYVTIP NMPIRFTKIF YNQQNHYDGS TGKFHCNIPG LYYFAYHITV YMKDVKVSLF KKDKAMLFTY DQYQENNVDQ ASGSVLLHLE VGDQVWLQVY GEGERNGLYA DNDNDSTFTG FLLYHDTN |
Source | Escherichia coli. |
Biological Activity | ED50 2× 10ˆ2 units/mg. |
Endotoxins | < 0.2 EU/μg, determined by LAL method. |
Storage | Lyophilized recombinant human Adiponectin (rhAdiponectin) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhAdiponectin remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. |
Buffer/Preservatives | Lyophilized after extensive dialysis against 50 mM Tris, 150 mM NaCl, pH 8.0. |
Alternative names | Acrp-30, Acrp-30, GBP-28, GBP28, Apm-1 Read more... |
Note | For research use only |
Application notes | Reconstituted in ddH2O at 100 μg/mL. |
Expiration Date | 6 months from date of receipt. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
Greater than 85% as determined by SDS-PAGE. | |
62.6 kDa | |
in vitro E.coli expression system |
Greater than 85% as determined by SDS-PAGE. | |
34.8 kDa | |
in vitro E.coli expression system |
Greater than 85% as determined by SDS-PAGE. | |
33.0 kDa | |
in vitro E.coli expression system |
CLIA, EIA, LF | |
> 95% as determined by SDS-PAGE. | |
30 kDa | |
HEK293 |
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