RCAN1 Antibody

• Catalog Number: orb1248717
• Category: Antibodies
• Description: RCAN1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Bovine, Canine, Human, Rat
• Reactivity: Mouse
• Immunogen: The immunogen for this antibody is: C-HIGSSHLAPPNPD
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40kDa band corresponding to isoform 1L observed in Mouse Brain lysates and this band is not present in the KO mouse. Additional bands around 55kDa were consistently observed, however these band were also observed in the KO mice and therefore they represent non-specific signal. We call for caution when used for other assays than Western blot. Data kindly provided by Dana Crawford, PhD, Albany Medical College, NY, USA. Recommended concentration: 1-3ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 40 kDa
• Target: RCAN1
• UniProt ID: P53805
• NCBI: NP_004405.3, NP_981963.1, NP_981962.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: RCAN1, regulator of calcineurin 1, ADAPT78, CSP1,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40kDa band corresponding to isoform 1L observed in Mouse Brain lysates and this band is not present in the KO mouse. Additional bands around 55kDa were consistently observed, however these band were also observed in the KO mice and therefore they represent non-specific signal. We call for caution when used for other assays than Western blot. Data kindly provided by Dana Crawford, PhD, Albany Medical College, NY, USA. Recommended concentration: 1-3ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248717 (1 ug/ml) staining of Mouse Brain and KO Mouse Brain lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.