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Catalog Number | orb1265034 |
---|---|
Category | Antibodies |
Description | RBX1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC-P, WB |
Predicted Reactivity | C. elegans, Mouse |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This RBX1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 74-108 amino acids from the C-terminal region of human RBX1. |
Concentration | batch dependent |
Dilution range | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:25For IF starting dilution is: 1:25 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 12 kDa |
Target | RBX1 |
UniProt ID | P62877 |
NCBI | P62877 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | E3 ubiquitin-protein ligase RBX1, 632-, Protein ZY Read more... |
Note | For research use only |
Application notes | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:25For IF starting dilution is: 1:25 |
Expiration Date | 12 months from date of receipt. |
Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 ug per lane.
Immunohistochemical analysis of paraffin-embedded M. testis section using RBX1 Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Fluorescent image of HeLa cells stained with RBX1 Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Immunohistochemical analysis of paraffin-embedded H. colon section using RBX1 Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from Hela, MCF-7 cell line (from left to right), using RBX1 Antibody at 1:1000 at each lane.
ICC, IHC-Fr, IHC-P, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
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